Pex11a deficiency causes dyslipidaemia and obesity in mice.

ABSTRACT: Peroxisomes play a central role in lipid metabolism. We previously demonstrated that Pex11a deficiency impairs peroxisome abundance and fatty acid β-oxidation and results in hepatic triglyceride accumulation. The role of Pex11a in dyslipidaemia and obesity is investigated here with Pex11a knockout mice (Pex11a^-/- ). Metabolic phenotypes including tissue weight, glucose tolerance, insulin sensitivity, cholesterol levels, fatty acid profile, oxygen consumption, physical activity were assessed in wild-type (WT) and Pex11a^-/- fed with a high-fat diet. Molecular changes and peroxisome abundance in adipose tissue were evaluated through qRT-PCR, Western blotting, and Immunofluorescence. Pex11a^-/- showed increased fat mass, decreased skeletal muscle, higher cholest

SUBMITTER: Chen C 

PROVIDER: S-EPMC6378206 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature