Organotypic slice culture based on in ovo electroporation for chicken embryonic central nervous system.

ABSTRACT: Organotypic slice culture is a living cell research technique which blends features of both in vivo and in vitro techniques. While organotypic brain slice culture techniques have been well established in rodents, there are few reports on the study of organotypic slice culture, especially of the central nervous system (CNS), in chicken embryos. We established a combined in ovo electroporation and organotypic slice culture method to study exogenous genes functions in the CNS during chicken embryo development. We performed in ovo electroporation in the spinal cord or optic tectum prior to slice culture. When embryonic development reached a specific stage, green fluorescent protein (GFP)-positive embryos were selected and fluorescent expression sites were cut under stereo fluorescence microsco

SUBMITTER: Yang C 

PROVIDER: S-EPMC6378233 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature