Abnormal Small Intestinal Epithelial Microvilli in Patients With Crohn's Disease.

ABSTRACT:

Background & aims

Crohn disease (CD) presents as chronic and often progressive intestinal inflammation, but the contributing pathogenic mechanisms are unclear. We aimed to identify alterations in intestinal cells that could contribute to the chronic and progressive course of CD.

Methods

We took an unbiased system-wide approach by performing sequence analysis of RNA extracted from formalin-fixed paraffin-embedded ileal tissue sections from patients with CD (n = 36) and without CD (controls; n = 32). We selected relatively uninflamed samples, based on histology, before gene expression profiling; validation studies were performed using adjacent serial tissue sections. A separate set of samples (3 control and 4 CD samples) was analyzed by transmission electron microscopy. We dev

SUBMITTER: VanDussen KL 

PROVIDER: S-EPMC6378688 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature