Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization.

ABSTRACT:

Background

The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis. However, it usually takes a long time to reach a high saccharification level using the current CBS biocatalyst and process.

Results

To promote the saccharification efficiency and reduce the cost, a Clostridium thermocellum recombinant strain ∆pyrF::KBm was constructed as a new CBS biocatalyst in this study. The key CBS factors, including the medium, inoculum size and cultivation, and substrate load, were i

SUBMITTER: Liu S 

PROVIDER: S-EPMC6378752 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature