Sperm capacitation is associated with phosphorylation of the testis-specific radial spoke protein Rsph6a†.

ABSTRACT: Mammalian sperm undergo a series of biochemical and physiological changes collectively known as capacitation in order to acquire the ability to fertilize. Although the increase in phosphorylation associated with mouse sperm capacitation is well established, the identity of the proteins involved in this signaling cascade remains largely unknown. Tandem mass spectrometry (MS/MS) has been used to identify the exact sites of phosphorylation and to compare the relative extent of phosphorylation at these sites. In the present work, we find that a novel site of phosphorylation on a peptide derived from the radial spoke protein Rsph6a is more phosphorylated in capacitated mouse sperm. The Rsph6a gene has six exons, five of which are conserved during evolution in flagellated cells. The exon contain

SUBMITTER: Paudel B 

PROVIDER: S-EPMC6378865 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature