Regulation of Ca²⁺ signaling by acute hypoxia and acidosis in cardiomyocytes derived from human induced pluripotent stem cells.

ABSTRACT:

Aims

The effects of acute (100 s) hypoxia and/or acidosis on Ca²⁺ signaling parameters of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are explored here for the first time.

Methods and results

1) hiPSC-CMs express two cell populations: rapidly-inactivating I_Ca myocytes (τ_i<40 ms, in 4-5 day cultures) and slowly-inactivating I_Ca (τ_i  ≥ 40 ms, in 6-8 day cultures). 2) Hypoxia suppressed I_Ca by 10-20% in rapidly- and 40-55% in slowly-inactivating I_Ca cells. 3) Isoproterenol enhanced I_Ca in hiPSC-CMs, but either enhanced or did not alter the hypoxic suppression. 4) Hypoxia had no differential suppressive effects in the two cell-types when Ba²⁺ was the charg

SUBMITTER: Fernandez-Morales JC 

PROVIDER: S-EPMC6378877 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature