Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells.

ABSTRACT: We describe droplet-assisted RNA targeting by single-cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.

SUBMITTER: Saikia M 

PROVIDER: S-EPMC6378878 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature