Deuterium induces a distinctive Escherichia coli proteome that correlates with the reduction in growth rate.

ABSTRACT: Substitution of protium (H) for deuterium (D) strongly affects biological systems. Whereas higher eukaryotes such as plants and mammals hardly survive a deuterium content of >30%, many microorganisms can grow on fully deuterated media, albeit at reduced rates. Very little is known about how the H/D replacement influences life at the systems level. Here, we used MS-based analysis to follow the adaptation of a large part of the Escherichia coli proteome from growth on a protonated full medium, over a protonated minimal medium, to a completely deuterated minimal medium. We could quantify >1800 proteins under all conditions, several 100 of which exhibited strong regulation during both adaptation processes. The adaptation to minimal medium strongly up-regulated amino acid synthesis and s

SUBMITTER: Opitz C 

PROVIDER: S-EPMC6378978 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature