Transcription factor TFAP2B up-regulates human corneal endothelial cell-specific genes during corneal development and maintenance.

ABSTRACT: The corneal endothelium, which originates from the neural crest via the periocular mesenchyme (PM), is crucial for maintaining corneal transparency. The development of corneal endothelial cells (CECs) from the neural crest is accompanied by the expression of several transcription factors, but the contribution of some of these transcriptional regulators to CEC development is incompletely understood. Here, we focused on activating enhancer-binding protein 2 (TFAP2, AP-2), a neural crest-expressed transcription factor. Using semiquantitative/quantitative RT-PCR and reporter gene and biochemical assays, we found that, within the AP-2 family, the TFAP2B gene is the only one expressed in human CECs in vivo and that its expression is strongly localized to the peripheral region of th

SUBMITTER: Hara S 

PROVIDER: S-EPMC6378988 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature