HOTMAQ: A Multiplexed Absolute Quantification Method for Targeted Proteomics.

ABSTRACT: Absolute quantification in targeted proteomics is challenging due to a variety of factors, including low specificity in complex backgrounds, limited analytical throughput, and wide dynamic range. To address these problems, we developed a hybrid offset-triggered multiplex absolute quantification (HOTMAQ) strategy that combines cost-effective mass difference and isobaric tags to enable simultaneous construction of an internal standard curve in the MS¹ precursor scan, real-time identification of peptides at the MS² level, and mass offset-triggered accurate quantification of target proteins in synchronous precursor selection (SPS)-MS³ spectra. This approach increases the analytical throughput of targeted quantitative proteomics by up to 12-fold. The HOTMAQ stra

SUBMITTER: Zhong X 

PROVIDER: S-EPMC6379083 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature