Functional Characterization of Two Putative DAHP Synthases of AroG1 and AroG2 and Their Links With Type III Secretion System in Ralstonia solanacearum.

ABSTRACT: Type three secretion system (T3SS) is essential for Ralstonia solanacearum to cause disease in host plants and we previously screened AroG1 as a candidate with impact on the T3SS expression. Here, we focused on two putative DAHP synthases of AroG1 and AroG2, which control the first step of the shikimate pathway, a common route for biosynthesis of aromatic amino acids (AAA), to characterize their functional roles and possible links with virulence in R. solanacearum. Deletion of aroG1/2 or aroG1, but not aroG2, significantly impaired the T3SS expression both in vitro and in planta, and the impact of AroG1 on T3SS was mediated with a well-characterized PrhA signaling cascade. Virulence of the aroG1/2 or aroG1 mutants was completel

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6379268 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature