Characterization of FlgP, an Essential Protein for Flagellar Assembly in Rhodobacter sphaeroides.

ABSTRACT: The flagellar lipoprotein FlgP has been identified in several species of bacteria, and its absence provokes different phenotypes. In this study, we show that in the alphaproteobacterium Rhodobacter sphaeroides, a ΔflgP mutant is unable to assemble the hook and the filament. In contrast, the membrane/supramembrane (MS) ring and the flagellar rod appear to be assembled. In the absence of FlgP a severe defect in the transition from rod to hook polymerization occurs. In agreement with this idea, we noticed a reduction in the amount of intracellular flagellin and the chemotactic protein CheY4, both encoded by genes dependent on σ²⁸ This suggests that in the absence of flgP the switch to export the anti-sigma factor, FlgM, does not occur. The presence of FlgP was

SUBMITTER: Perez-Gonzalez C 

PROVIDER: S-EPMC6379573 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature