Finely tuned conformational dynamics regulate the protective function of the lncRNA MALAT1 triple helix.

ABSTRACT: Nucleic acid triplexes may regulate many important biological processes. Persistent accumulation of the oncogenic 7-kb long noncoding RNA MALAT1 is dependent on an unusually long intramolecular triple helix. This triplex structure is positioned within a conserved ENE (element for nuclear expression) motif at the lncRNA 3' terminus and protects the entire transcript from degradation in a polyA-independent manner. A requisite 3' maturation step leads to triplex formation though the precise mechanism of triplex folding remains unclear. Furthermore, the contributions of several peripheral structural elements to triplex formation and protective function have not been determined. We evaluated the stability, conformational fluctuations, and function of this MALAT1 ENE triple helix (M1TH) protecti

SUBMITTER: Ageeli AA 

PROVIDER: S-EPMC6379651 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature