Characterizing the nuclease accessibility of DNA in human cells to map higher order structures of chromatin.

ABSTRACT: Packaging of DNA into chromatin regulates DNA accessibility and consequently all DNA-dependent processes. The nucleosome is the basic packaging unit of DNA forming arrays that are suggested, by biochemical studies, to fold hierarchically into ordered higher-order structures of chromatin. This organization has been recently questioned using microscopy techniques, proposing an irregular structure. To address the principles of chromatin organization, we applied an in situ differential MNase-seq strategy and analyzed in silico the results of complete and partial digestions of human chromatin. We investigated whether different levels of chromatin packaging exist in the cell. We assessed the accessibility of chromatin within distinct domains of kb to Mb genomic regions, performed statistical ana

SUBMITTER: Schwartz U 

PROVIDER: S-EPMC6379673 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature