Single-nucleotide-resolution mapping of DNA gyrase cleavage sites across the Escherichia coli genome.

ABSTRACT: An important antibiotic target, DNA gyrase is an essential bacterial enzyme that introduces negative supercoils into DNA and relaxes positive supercoils accumulating in front of moving DNA and RNA polymerases. By altering the superhelical density, gyrase may regulate expression of bacterial genes. The information about how gyrase is distributed along genomic DNA and whether its distribution is affected by drugs is scarce. During catalysis, gyrase cleaves both DNA strands forming a covalently bound intermediate. By exploiting the ability of several topoisomerase poisons to stabilize this intermediate we developed a ChIP-Seq-based approach to locate, with single nucleotide resolution, DNA gyrase cleavage sites (GCSs) throughout the Escherichia coli genome. We identified an extended gyrase bi

SUBMITTER: Sutormin D 

PROVIDER: S-EPMC6379681 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature