RNA-seq based transcriptional analysis of Saccharomyces cerevisiae and Lachancea thermotolerans in mixed-culture fermentations under anaerobic conditions.

ABSTRACT:

Background

In wine fermentation starter cultures, the blending of non-Saccharomyces yeast with Saccharomyces cerevisiae to improve the complexity of wine has become common practice, but data regarding the impact of co-cultivation on yeast physiology and on genetic and metabolic regulation remain limited. Here we describe a transcriptomic analysis of mixed fermentations of Saccharomyces cerevisiae and Lachancea thermotolerans. The fermentations were carried out in carefully controlled environmental conditions in a bioreactor to reduce transcriptomic responses that would be due to factors other than the presence of the second species.

Results

The transcriptomic data revealed that both yeast species showed a clear response to the presence of the other. Affected genes primarily

SUBMITTER: Shekhawat K 

PROVIDER: S-EPMC6379982 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature