RNA-Seq reveals new DELLA targets and regulation in transgenic GA-insensitive grapevines.

ABSTRACT:

Background

Gibberellins (GAs) and their regulator DELLA are involved in many aspects of plant growth and development and most of our current knowledge in the DELLA-facilitated GA signaling was obtained from the studies of annual species. To understand GA-DELLA signaling in perennial species, we created ten GA-insensitive transgenic grapevines carrying a DELLA mutant allele (Vvgai1) in the background of Vitis vinifera 'Thompson Seedless' and conducted comprehensive analysis of their RNA expression profiles in the shoot, leaf and root tissues.

Results

The transgenic lines showed varying degrees of dwarf stature and other typical DELLA mutant phenotypes tightly correlated with the levels of Vvgai1 expression. A large number of differentially expressed genes (DEGs) were identifi

SUBMITTER: Arro J 

PROVIDER: S-EPMC6379989 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature