N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei.

ABSTRACT:

Background

The filamentous fungus Trichoderma reesei produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. N,N-dimethylformamide (DMF) is a versatile organic solvent used in large quantities in industries.

Results

In this study, we serendipitously found that biologically relevant concentrations of extracellular DMF-induced cellulase production in the T. reesei hyper-cellulolytic mutant Rut-C30 and wild-type strain QM6a. Next, by transcriptome analysis, we determined that plc-e encoding phospholipase C was activated by DMF and revealed that cytosolic Ca²⁺ plays a vital role in the response of T. reesei to DMF. Using EGTA (a putative extracellular Ca²⁺ chelator) and

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6380019 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature