Major contribution of transcription initiation to 5'-end formation of mitochondrial steady-state transcripts in maize.

ABSTRACT: In plant mitochondria, some steady-state transcripts contain primary 5' ends derived from transcription initiation, while the others have processed 5' termini generated by post-transcriptional processing. Differentiation and mapping of the primary and processed transcripts are important for unraveling the molecular mechanism(s) underlying transcription and transcript end maturation. However, previous efforts to systematically differentiate these two types of transcripts in plant mitochondria failed. At present, it is considered that the majority of mature mRNAs may have processed 5' ends in Arabidopsis. Here, by combination of circular RT-PCR, quantitative RT-PCR, RNA 5'-polyphosphatase treatment and Northern blot, we successfully discriminated and mapped the primary and processed transcri

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6380311 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature