In vitro and in silico assessment of the developability of a designed monoclonal antibody library.

ABSTRACT: Despite major advances in antibody discovery technologies, the successful development of monoclonal antibodies (mAbs) into effective therapeutic and diagnostic agents can often be impeded by developability liabilities, such as poor expression, low solubility, high viscosity and aggregation. Therefore, strategies to predict at the early phases of antibody development the risk of late-stage failure of antibody candidates are highly valuable. In this work, we employ the in silico solubility predictor CamSol to design a library of 17 variants of a humanized mAb predicted to span a broad range of solubility values, and we examine their developability potential with a battery of commonly used in vitro and in silico assays. Our results demonstrate the ability of CamSol to rationally enhance mAb d

SUBMITTER: Wolf Perez AM 

PROVIDER: S-EPMC6380425 | biostudies-literature | 2019 Feb/Mar

REPOSITORIES: biostudies-literature