Single gold-bridged nanoprobes for identification of single point DNA mutations.

ABSTRACT: Consensus ranking of protein affinity to identify point mutations has not been established. Therefore, analytical techniques that can detect subtle variations without interfering with native biomolecular interactions are required. Here we report a rapid method to identify point mutations by a single nanoparticle sensing system. DNA-directed gold crystallization forms rod-like nanoparticles with bridges based on structural design. The nanoparticles enhance Rayleigh light scattering, achieving high refractive-index sensitivity, and enable the system to monitor even a small number of protein-DNA binding events without interference. Analysis of the binding affinity can compile an atlas to distinguish the potential of various point mutations recognized by MutS protein. We use the atlas to analy

SUBMITTER: Ma X 

PROVIDER: S-EPMC6381086 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature