Identification of Subject-Specific Immunoglobulin Alleles From Expressed Repertoire Sequencing Data.

ABSTRACT: The adaptive immune receptor repertoire (AIRR) contains information on an individuals' immune past, present and potential in the form of the evolving sequences that encode the B cell receptor (BCR) repertoire. AIRR sequencing (AIRR-seq) studies rely on databases of known BCR germline variable (V), diversity (D), and joining (J) genes to detect somatic mutations in AIRR-seq data via comparison to the best-aligning database alleles. However, it has been shown that these databases are far from complete, leading to systematic misidentification of mutated positions in subsets of sample sequences. We previously presented TIgGER, a computational method to identify subject-specific V gene genotypes, including the presence of novel V gene alleles, directly from AIRR-seq data. However, the original

SUBMITTER: Gadala-Maria D 

PROVIDER: S-EPMC6381938 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature