Matrix Rigidity-Dependent Regulation of Ca²⁺ at Plasma Membrane Microdomains by FAK Visualized by Fluorescence Resonance Energy Transfer.

ABSTRACT: The dynamic regulation of signal transduction at plasma membrane microdomains remains poorly understood due to limitations in current experimental approaches. Genetically encoded biosensors based on fluorescent resonance energy transfer (FRET) can provide high spatiotemporal resolution for imaging cell signaling networks. Here, distinctive regulation of focal adhesion kinase (FAK) and Ca²⁺ signals are visualized at different membrane microdomains by FRET using membrane-targeting biosensors. It is shown that rigidity-dependent FAK and Ca²⁺ signals in human mesenchymal stem cells (hMSCs) are selectively activated at detergent-resistant membrane (DRM or rafts) microdomains during the cell-matrix adhesion process, with minimal activities at non-DRM domains. The rigidity-d

SUBMITTER: Kim TJ 

PROVIDER: S-EPMC6382294 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature