Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer.

ABSTRACT: Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multidisciplinary approach using confocal and electron microscopy, detection of Ca2+, callose, and cellulose, treatments with caffeine, digitonin, and endosidin7, morphometry, qPCR, osmometry, and viability assays in order to study the dynamics of cell wall formation during embryogenesis induction in a high-response rapeseed (Brassica napus) line and two recalcitrant rapeseed and eggplant (Solanum melongena) lines. Formation of a callose-rich subintinal layer (SL) was common to microspore embryogenesis in the different genotypes. However, this process was directly related

SUBMITTER: Rivas-Sendra A 

PROVIDER: S-EPMC6382338 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature