Cochlear outer hair cell horizontal top connectors mediate mature stereocilia bundle mechanics.

ABSTRACT: Outer hair cell (OHC) stereocilia bundle deflection opens mechanoelectrical transduction channels at the tips of the stereocilia from the middle and short rows, while bundle cohesion is maintained owing to the presence of horizontal top connectors. Here, we used a quantitative noncontact atomic force microscopy method to investigate stereocilia bundle stiffness and damping, when stimulated at acoustic frequencies and nanometer distances from the bundle. Stereocilia bundle mechanics were determined in stereocilin-deficient mice lacking top connectors and with detached tectorial membrane (Strc ^-/-/Tecta ^-/- double knockout) and heterozygous littermate controls (Strc ^+/-/Tecta ^-/-). A substantial decrease in bundle stiffn

SUBMITTER: Cartagena-Rivera AX 

PROVIDER: S-EPMC6382404 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature