The AAA+ protease ClpXP can easily degrade a 3₁ and a 5₂-knotted protein.

ABSTRACT: Knots in proteins are hypothesized to make them resistant to enzymatic degradation by ATP-dependent proteases and recent studies have shown that whereas ClpXP can easily degrade a protein with a shallow 3₁ knot, it cannot degrade 5₂-knotted proteins if degradation is initiated at the C-terminus. Here, we present detailed studies of the degradation of both 3₁- and 5₂-knotted proteins by ClpXP using numerous constructs where proteins are tagged for degradation at both N- and C-termini. Our results confirm and extend earlier work and show that ClpXP can easily degrade a deeply 3₁-knotted protein. In contrast to recently published work on the degradation of 5₂-knotted proteins, our results show that the ClpXP machinery can also

SUBMITTER: Sivertsson EM 

PROVIDER: S-EPMC6382783 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature