SDS-induced oligomerization of Lys49-phospholipase A₂ from snake venom.

ABSTRACT: Phospholipase A₂ (PLA₂) is one of the representative toxic components of snake venom. PLA₂s are categorized into several subgroups according to the amino acid at position 49, which comprises either Asp49, Lys49, Arg49 or Ser49. Previous studies suggested that the Lys49-PLA₂ assembles into an extremely stable dimer. Although the behavior on Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing or non-reducing conditions suggested the presence of intermolecular disulfide bonds, these bonds were not observed in the crystal structure of Lys49-PLA₂. The reason for this discrepancy between the crystal structure and SDS-PAGE of Lys49-PLA₂ remains unknown. In this study, we analyzed a Lys49-PLA₂

SUBMITTER: Matsui T 

PROVIDER: S-EPMC6382788 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature