Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves.

ABSTRACT: Cytosolic lipid droplets are endoplasmic reticulum-derived organelles typically found in seeds as reservoirs for physiological energy and carbon to fuel germination. Here, we report synthetic biology approaches to co-produce high-value sesqui- or diterpenoids together with lipid droplets in plant leaves. The formation of cytosolic lipid droplets is enhanced in the transient Nicotiana benthamiana system through ectopic production of WRINKLED1, a key regulator of plastid fatty acid biosynthesis, and a microalgal lipid droplet surface protein. Engineering of the pathways providing the universal C5-building blocks for terpenoids and installation of terpenoid biosynthetic pathways through direction of the enzymes to native and non-native compartments boost the production of target terpenoids. W

SUBMITTER: Sadre R 

PROVIDER: S-EPMC6382807 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature