Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein.

ABSTRACT: miniSOG is the first flavin-binding protein that has been developed with the specific aim of serving as a genetically-encodable light-induced source of singlet oxygen (¹O₂). We have determined its 1.17 Å resolution structure, which has allowed us to investigate its mechanism of photosensitization using an integrated approach combining spectroscopic and structural methods. Our results provide a structural framework to explain the ability of miniSOG to produce ¹O₂ as a competition between oxygen- and protein quenching of its triplet state. In addition, a third excited-state decay pathway has been identified that is pivotal for the performance of miniSOG as ¹O₂ photosensitizer, namely the photo-induced transformation of flavin

SUBMITTER: Torra J 

PROVIDER: S-EPMC6382843 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature