Top-down Mass Spectrometry Analysis of Human Serum Autoantibody Antigen-Binding Fragments.

ABSTRACT: Detecting autoimmune diseases at an early stage is crucial for effective treatment and disease management to slow disease progression and prevent irreversible organ damage. In many autoimmune diseases, disease-specific autoantibodies are produced by B cells in response to soluble autoantigens due to defects in B cell tolerance mechanisms. Autoantibodies accrue early in disease development, and several are so disease-specific they serve as classification criteria. In this study, we established a high-throughput, sensitive, intact serum autoantibody analysis platform based on the optimization of a one dimensional ultra-high-pressure liquid chromatography top-down mass spectrometry platform (1D UPLC-TDMS). This approach has been successfully applied to a 12 standard monoclonal antibody antige

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6382847 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature