Variable 3'polyadenylation of Wheat yellow mosaic virus and its novel effects on translation and replication.

ABSTRACT:

Background

Polyadenylation influences many aspects of mRNA as well as viral RNA. variable polyadenylation at the 3' end have been reported in RNA viruses. It is interesting to identify the characteristic and potential role of 3' polyadenylation of Wheat yellow mosaic virus (WYMV), which has been reported to contain two genomic RNAs with 3' poly(A) tails and caused severe disease on wheat in East Asia region.

Methods

3' RACE was used to identify sequences of the 3' end in WYMV RNAs from naturally infected wheat by WYMV. In vitro translation assay was performed to analyze effect of UTRs of WYMV with or without 3'polyadenylation on translation. In vitro replication mediated by WYMV NIb protein were performed to evaluate effect of variable polyadenylation on replication.

Resu

SUBMITTER: Geng G 

PROVIDER: S-EPMC6383263 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature