Mechanism for Regulation of Melanoma Cell Death via Activation of Thermo-TRPV4 and TRPV2.

ABSTRACT:

Background

Thermo-TRPs (temperature-sensitive transient receptor potential channels) belong to the TRP (transient receptor potential) channel superfamily. Emerging evidence implied that thermo-TRPs have been involved in regulation of cell fate in certain tumors. However, their distribution profiles and roles in melanoma remain incompletely understood.

Methods

Western blot and digital PCR approaches were performed to identify the distribution profiles of six thermo-TRPs. MTT assessment was employed to detect cell viability. Flow cytometry was applied to test cell cycle and apoptosis. Calcium imaging was used to determine the function of channels. Five cell lines, including one normal human primary epidermal melanocytes and two human malignant melanoma (A375, G361) and two hum

SUBMITTER: Zheng J 

PROVIDER: S-EPMC6383420 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature