Characterizing a mouse model for evaluation of countermeasures against hydrogen sulfide-induced neurotoxicity and neurological sequelae.

ABSTRACT: Hydrogen sulfide (H₂ S) is a highly neurotoxic gas. It is the second most common cause of gas-induced deaths. Beyond mortality, surviving victims of acute exposure may suffer long-term neurological sequelae. There is a need to develop countermeasures against H₂ S poisoning. However, no translational animal model of H₂ S-induced neurological sequelae exists. Here, we describe a novel mouse model of H₂ S-induced neurotoxicity for translational research. In paradigm I, C57/BL6 mice were exposed to 765 ppm H₂ S for 40 min on day 1, followed by 15-min daily exposures for periods ranging from 1 to 6 days. In paradigm II, mice were exposed once to 1000 ppm H₂ S for 60 minutes. Mice were assessed for behavioral, neurochemical, bioch

SUBMITTER: Anantharam P 

PROVIDER: S-EPMC6383676 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature