Hole Hopping Across a Protein-Protein Interface.

ABSTRACT: We have investigated photoinduced hole hopping in a Pseudomonas aeruginosa azurin mutant Re126WWCu^I, where two adjacent tryptophan residues (W124 and W122) are inserted between the Cu^I center and a Re photosensitizer coordinated to a H126 imidazole (Re = Re^I(H126)(CO)₃(dmp)⁺, dmp = 4,7-dimethyl-1,10-phenanthroline). Optical excitation of this mutant in aqueous media (≤40 μM) triggers 70 ns electron transport over 23 Å, yielding a long-lived (120 μs) Re^I(H126)(CO)₃(dmp^•-)WWCu^II product. The Re126FWCu^I mutant (F124, W122) is not redox-active under these conditions. Upon increasing the concentration to 0.2-2 mM, {Re126WWCu^I}₂ and {Re126FWCu^I}₂

SUBMITTER: Takematsu K 

PROVIDER: S-EPMC6384139 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature