Exploring the suitability of RanBP2-type Zinc Fingers for RNA-binding protein design.

ABSTRACT: Transcriptomes consist of several classes of RNA that have wide-ranging but often poorly described functions and the deregulation of which leads to numerous diseases. Engineering of functionalized RNA-binding proteins (RBPs) could therefore have many applications. Our previous studies suggested that the RanBP2-type Zinc Finger (ZF) domain is a suitable scaffold to investigate the design of single-stranded RBPs. In the present work, we have analyzed the natural sequence specificity of various members of the RanBP2-type ZF family and characterized the interaction with their target RNA. Surprisingly, our data showed that natural RanBP2-type ZFs with different RNA-binding residues exhibit a similar sequence specificity and therefore no simple recognition code can be established. Despite this f

SUBMITTER: De Franco S 

PROVIDER: S-EPMC6384913 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature