Enhanced L-methionine production by genetically engineered Escherichia coli through fermentation optimization.

ABSTRACT: Microbial fermentation for L-methionine (L-Met) production based on natural renewable resources is attractive and challenging. In this work, the effects of medium composition and fermentation conditions were investigated to improve L-Met production by genetically engineered Escherichia coli MET-3. Statistical optimization techniques including Plackett-Burman (PB) design and Box-Behnken design (BBD) were adopted first to optimize the culture medium. Results of PB-designed experiments indicated that the culture medium components including glucose, yeast extract, KH₂PO₄, and MgSO₄.7H₂O had significant effects on L-Met biosynthesis. With their best-predicted concentration established by BBD (glucose 37.43 g/L, yeast extract 0.95 g/L, KH_2<

SUBMITTER: Zhou HY 

PROVIDER: S-EPMC6385071 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature