Measuring Ultra-Weak Protein Self-Association by Non-ideal Sedimentation Velocity.

ABSTRACT: Ultra-weak self-association can govern the macroscopic solution behavior of concentrated macromolecular solutions ranging from food products to pharmaceutical formulations and the cytosol. For example, it can promote dynamic assembly of multi-protein signaling complexes, lead to intracellular liquid-liquid phase transitions, and seed crystallization or pathological aggregates. Unfortunately, weak self-association is technically extremely difficult to study, as it requires very high protein concentrations where short intermolecular distances cause strongly correlated particle motion. Additionally, protein samples near their solubility limit in vitro frequently show some degree of polydispersity. Here we exploit the strong mass-dependent separation of assemblies in the centrifugal field to s

SUBMITTER: Chaturvedi SK 

PROVIDER: S-EPMC6385077 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature