Coupling of a viral K⁺-channel with a glutamate-binding-domain highlights the modular design of ionotropic glutamate-receptors.

ABSTRACT: Ionotropic glutamate receptors (iGluRs) mediate excitatory neuronal signaling in the mammalian CNS. These receptors are critically involved in diverse physiological processes; including learning and memory formation, as well as neuronal damage associated with neurological diseases. Based on partial sequence and structural similarities, these complex cation-permeable iGluRs are thought to descend from simple bacterial proteins emerging from a fusion of a substrate binding protein (SBP) and an inverted potassium (K⁺)-channel. Here, we fuse the pore module of the viral K⁺-channel Kcv_ATCV-1 to the isolated glutamate-binding domain of the mammalian iGluR subunit GluA1 which is structural homolog to SBPs. The resulting chimera (GluATCV*) is functional and display

SUBMITTER: Schonrock M 

PROVIDER: S-EPMC6385376 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature