Glycerol kinase 5 confers gefitinib resistance through SREBP1/SCD1 signaling pathway.

ABSTRACT:

Background

Drug resistance is common in cancer chemotherapy. This study investigates the role of Glycerol kinase 5 (GK5) in mediating gefitinib resistance in NSCLC.

Methods

The exosomal mRNA of GK5 was detected using a tethered cationic lipoplex nanoparticle (TCLN) biochip. Real-time PCR and Western blot were used to examine the expression of GK5 mRNA and protein in gefitinib-sensitive and -resistant human lung adenocarcinoma cells. The cell counting kit-8, EdU assay, flow cytometry, and JC-1 dye were used to measure cell proliferation, cell cycle, and the mitochondrial membrane potential.

Results

We found that the exosomal mRNA of GK5 in the plasma of patients with gefitinib-resistant adenocarcinoma was significantly higher compared with that of gefitinib-sensitive p

SUBMITTER: Zhou J 

PROVIDER: S-EPMC6385389 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature