MiR-9 promotes tumorigenesis and angiogenesis and is activated by MYC and OCT4 in human glioma.

ABSTRACT:

Background

Glioma, characterized by its undesirable prognosis and poor survival rate, is a serious threat to human health and lives. MicroRNA-9 (miR-9) is implicated in the regulation of multiple tumors, while the mechanisms underlying its aberrant expression and functional alterations in human glioma are still controversial.

Methods

Expressions of miR-9 were measured in GEO database, patient specimens and glioma cell lines. Gain- and loss-of-function assays were applied to identify the effects of miR-9 on glioma cells and HUVECs in vitro and in vivo. Potential targets of miR-9 were predicted by bioinformatics and further verified via in vitro experiments. Transcriptional regulation of miR-9 by MYC and OCT4 was determined in glioma cells.

Results

MiR-9 was frequently

SUBMITTER: Chen X 

PROVIDER: S-EPMC6385476 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature