The glial sodium-potassium-2-chloride cotransporter is required for synaptic transmission in the Drosophila visual system.

ABSTRACT: The Drosophila Ncc69 gene encodes a Na⁺-K⁺-2Cl^--cotransporter (NKCC) that is critical for regulating intra- and extracellular ionic conditions in different tissues. Here, we show that the Ncc69 transporter is necessary for fly vision and that its expression is required non-autonomously in glia to maintain visual synaptic transmission. Flies mutant for Ncc69 exhibit normal photoreceptor depolarization in response to a light pulse but lack the ON and OFF-transients characteristic of postsynaptic responses of lamina neurons, indicating a failure in synaptic transmission. We also find that synaptic transmission requires the Ncc69 regulatory kinases WNK and Fray in glia. The ERG phenotype is associated with a defect in the recycling of the histamine neurotransmi

SUBMITTER: Stenesen D 

PROVIDER: S-EPMC6385505 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature