Neurocytometry: Flow Cytometric Sorting of Specific Neuronal Populations from Human and Rodent Brain.

ABSTRACT: Flow cytometry has the potential to facilitate understanding of the heterogeneous responses of diverse brain cell populations to a variety of stimuli. However, existing methods of applying flow cytometry to brain tissues are each limited in certain ways. They either require genetically labeled cells to achieve separation of specific populations, are not applicable to previously fixed tissue, or are not compatible with downstream mRNA analysis. Here, we describe a group of related methods that overcome many previous limitations and allow robust sorting and downstream molecular analysis of highly enriched populations of specific neuronal and non-neuronal cells from any mammalian brain. We illustrate these techniques, which are compatible with antibodies for both nuclear and non-nuclear epito

SUBMITTER: Martin D 

PROVIDER: S-EPMC6385603 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature