Bone healing in an aged murine fracture model is characterized by sustained callus inflammation and decreased cell proliferation.

ABSTRACT: Geriatric fractures take longer to heal and heal with more complications than those of younger patients; however, the mechanistic basis for this difference in healing is not well understood. To improve this understanding, we investigated cell and molecular differences in fracture healing between 5-month-old (young adult) and 25-month-old (geriatric) mice healing utilizing high-throughput analysis of gene expression. Mice underwent bilateral tibial fractures and fracture calluses were harvested at 5, 10, and 20 days post-fracture (DPF) for analysis. Global gene expression analysis was performed using Affymetrix MoGene 1.0 ST microarrays. After normalization, data were compared using ANOVA and evaluated using Principal Component Analysis (PCA), CTen, heatmap, and Incromaps analysis. PCA and

SUBMITTER: Hebb JH 

PROVIDER: S-EPMC6385606 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature