Guanidine thiocyanate solution facilitates sample collection for plant rhizosphere microbiome analysis.

ABSTRACT: The interactions between rhizosphere microorganisms and plants are important for the health and development of crops. Analysis of plant rhizosphere bacterial compositions, particularly of those with resistance to biotic/abiotic stresses, may improve their applications in sustainable agriculture. Large-scale rhizosphere samplings in the field are usually required; however, such samples, cannot be immediately frozen. We found that the storage of samples at room temperature for 2 days leads to a considerable reduction in the operational taxonomic unit (OTU) number and the indices of bacterial alpha-diversity of rhizosphere communities. In this study, in order to overcome these problems, we established a method using guanidine thiocyanate (GTC) solution for the preservation of rhizosphere samp

SUBMITTER: Sun X 

PROVIDER: S-EPMC6385689 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature