Spatiotemporal quantification of acoustic cell patterning using Voronoi tessellation.

ABSTRACT: Acoustic patterning using ultrasound standing waves has recently emerged as a potent biotechnology enabling the remote generation of ordered cell systems. This capability has opened up exciting opportunities, for example, in guiding the development of organoid cultures or the organization of complex tissues. The success of these studies is often contingent on the formation of tightly-packed and uniform cell arrays; however, a number of factors can act to disrupt or prevent acoustic patterning. Yet, to the best of our knowledge, there has been no comprehensive assessment of the quality of acoustically-patterned cell populations. In this report we use a mathematical approach, known as Voronoï tessellation, to generate a series of metrics that can be used to measure the effect of cell concent

SUBMITTER: Armstrong JPK 

PROVIDER: S-EPMC6386121 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature