Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning.

ABSTRACT: Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a w

SUBMITTER: Armstrong JPK 

PROVIDER: S-EPMC6386124 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature