Protein expression and function of organic anion transporters in short-term and long-term cultures of Huh7 human hepatoma cells.

ABSTRACT: Human-derived hepatic cell lines are a valuable alternative to primary hepatocytes for drug metabolism, transport and toxicity studies. However, their relevance for investigations of drug-drug and drug-organic anion (e.g., bile acid, steroid hormone) interactions at the transporter level remains to be established. The aim of the present study was to determine the suitability of the Huh7 cell line for transporter-dependent experiments. Huh7 cells were cultured for 1 to 4 weeks and subsequently were analyzed for protein expression, localization and activity of solute carrier (SLC) and ATP-binding cassette (ABC) transporters involved in organic anion transport using liquid chromatography-tandem mass spectroscopy, immunocytochemistry, and model substrates [³H]taurocholate (TCA), [

SUBMITTER: Malinen MM 

PROVIDER: S-EPMC6386193 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature