Little cigars and cigarillos harbor diverse bacterial communities that differ between the tobacco and the wrapper.

ABSTRACT: Despite their potential importance with regard to infectious and chronic diseases among tobacco users, microbial constituents of tobacco products lack characterization. Specifically, to our knowledge, there are no data describing the bacterial diversity of little cigars or cigarillos. To address this knowledge gap, we tested four brands of little cigars and cigarillos. Tobacco and wrapper subsamples (n = 132) were separately subjected to DNA extraction, followed by PCR amplification of the V3V4 hypervariable region of the 16S rRNA gene, and sequencing using Illumina HiSeq. Sequences were analyzed using QIIME and Phyloseq implemented in R. We identified 2,681 operational taxonomic units across all products. Significant differences in alpha and beta diversity were observed between Swisher Sw

SUBMITTER: Chattopadhyay S 

PROVIDER: S-EPMC6386278 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature