The C-terminus of the retinal homeobox (rax) gene product modulates transcription in a context-dependent manner.

ABSTRACT:

Purpose

The evolutionarily conserved retinal homeobox (Rax) transcription factor is essential for normal eye development in all vertebrates. Despite Rax's biologic significance, the molecular mechanisms underlying Rax molecular function as a transcriptional regulator are poorly defined. The rax gene encodes a conserved octapeptide motif (OP) near the N-terminus and several conserved regions in the C-terminus of unknown function, including the orthopedia, aristaless, rax (OAR) domain and the RX domain. The purpose of this study is to investigate the contribution of these conserved domains in Rax function.

Methods

N-and C-terminal deletion and point mutations were generated in Xenopus laevis rax.L (previously known as Rx1A) using PCR-based methods. We examined th

SUBMITTER: Buescher JL 

PROVIDER: S-EPMC6386513 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature