Salmonella Fimbrial Protein FimH Is Involved in Expression of Proinflammatory Cytokines in a Toll-Like Receptor 4-Dependent Manner.

ABSTRACT: Type 1 fimbriae are proteinaceous filamentous structures present on bacterial surfaces and are mainly composed of the major fimbrial protein subunit FimA and the adhesive protein FimH, which is located at the tip of the fimbrial shaft. Here, we investigated the involvement of type 1 fimbriae in the expression of proinflammatory cytokines in macrophages infected with Salmonella enterica serovar Typhimurium. The level of interleukin-1β (IL-1β) mRNA was lower in macrophages infected with fimA or fimH mutant strains than in those infected with wild-type Salmonella Treatment of macrophages with purified recombinant FimH protein, but not FimA, resulted in the activation of the mitogen-activated protein kinase and nuclear factor κB signaling pathways, leading to the ex

SUBMITTER: Uchiya KI 

PROVIDER: S-EPMC6386536 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature